Rev
Tat
Vif
Integrase
Proteomics
Env
Nef

Rev

Unspliced and partially-spliced viral mRNAs encoding structural proteins and genomic RNA to be packaged into virions are exported from the nucleus to the cytoplasm by the regulatory protein Rev. This activity involves the binding of Rev subunits to the Rev Response Element (RRE) located in an intronic region of the unspliced transcripts. Rev then interacts with the host protein Crm1 (Exportin 1), forming a complex with GTP-bound Ran. This entire RNA-protein complex is then moved through the nuclear pore and released in the cytoplasm. The Rev project, headed by Dr. Alan Frankel, is aimed at determining structures of Rev in complex with its various binding partners, using NMR, crystallography and cryo-EM, with the goal of understanding the Rev-RRE oligomeric complex and its interactions with the CRM1 export receptor.