Throughout the HIV life cycle, viral-host complexes play an integral role in the biology of the virus. Assemblies range from binary protein-protein and protein-nucleic acid complexes to much larger multicomponent complexes. High-resolution structures of these complexes offer the potential for targeted intervention strategies in the treatment of AIDS.
The HARC Center has taken a broad systems-to-structure approach to define the HIV-host interactome and explore six major projects. Our projects focus on three main biological themes: Evasion of anti-viral restriction factors and manipulation of host cell function through degradation pathways (Vif, Vpu, Vpr and PR); HIV hijacking of host machineries for handling nucleic acids: transcription, nuclear export, RNA trafficking, and packaging (Tat and Rev); as well as membrane-associated machineries (Vpu and Nef). The Center also has four Technology Cores--Cryo-EM, Proteomics/Genomics, Computational Structural Biology, and Virology--required for efficient execution of all the Center projects.