Functional Proteomics Core

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Core Leader: Nevan Krogan

The Functional Proteomics Core has pioneered methods to establish a more comprehensive landscape of interactions, including developments in proteomic analysis, particularly with respect to post-translational modifications that can expand the functional and structural repertoire of HIV protein complexes. We are globally defining sets of validated host factors involved in different stages of HIV pathogenesis and are providing new targets for structural characterization.

This core will apply mass spectrometry-based proteomics approaches to characterize HIV-host interactions and associated changes to cellular protein abundance, posttranslational modifications (PTMs) and protein-protein interactions (PPIs) by: characterizing global protein and post-translational modification (PTM) response to HIV infection in primary T-cells, providing affinity purification (AP-MS) and proximity based biotinylation (APEX-MS) capabilities for studying HIV-host interactions, and determining structures and interaction networks of virus-host complexes by cross-linking mass spectrometry (XL-MS).